I-Mole Lab provides advanced genomics services aimed at decoding, analyzing,and interpreting DNA-level information across a wide range of organisms.

Our genomics workflows are designed to support population genetics, functional
genomics, evolutionary studies, and molecular biology applications.
1. NGS Analysis
• Comprehensive processing of raw sequencing data, including base quality assessment,
read trimming, and alignment to reference genomes.
• Detection and annotation of genetic variants such as SNPs, INDELs, copy number
variations (CNVs), and structural variants (SVs).
• Comparative genomics analysis across samples or populations to identify genetic
diversity and disease-associated variants.
2. Genome Annotation
• Structural annotation to identify genes, coding regions, non-coding RNAs, and
regulatory elements.
• Functional annotation using curated databases to assign gene functions, protein
domains, and biological pathways.
• Comparative annotation for newly sequenced genomes.
3. CRISPR Design
• Design and optimization of guide RNAs (gRNAs) targeting specific genomic loci.
• Prediction and minimization of off-target effects.
• Evaluation of editing efficiency and target accessibility.
4. In-Silico Cloning
• Simulation of cloning strategies including vector selection and insert integration.
• Verification of restriction enzyme sites, reading frames, and construct orientation.
• Generation of cloning maps ready for laboratory implementation.
5. siRNA & RNAi Design
• Rational design of gene-silencing molecules based on sequence specificity.
• Efficiency prediction and off-target risk assessment.
• Optimization for experimental or therapeutic applications.
6. Primer Design
• Design of primers optimized for specificity, melting temperature, and GC content.
• Validation for PCR, qPCR, and sequencing-based applications.
7. Phylogenetic Tree Analysis
• Construction of phylogenetic trees using multiple algorithms.
• Evolutionary and comparative analysis of genes, proteins, or organisms.